cell suspensions  (MedChemExpress)

Journal: Science Advances

Article Title: Space-time acoustofluidic tweezers for dynamic and selective manipulation of microparticles

doi: 10.1126/sciadv.aee2983

Figure Lengend Snippet: ( A ) Working principle of STAT. (i) Two orthogonal standing SAW fields with slightly detuned frequencies, ω x and ω y , pattern PACPs (red) and NACPs (blue) in distinct pressure regions; inset: photo of the STAT chip. (ii) Pressure-field evolution over one detuning cycle (0 to 2π/Δω), showing dynamic shifts of pressure nodes and antinodes induced by the frequency difference Δω. (iii) Schematic of the dynamic, static, and drag forces acting on PACPs and NACPs. ( B ) (i) High-throughput, low-frequency, shear-like oscillation of PACPs under the dynamic force field distribution. (ii) Time-lapse images over one oscillation cycle with 10-μm polystyrene beads in water. ( C ) (i) High-throughput, low-frequency, longitudinal-like oscillation of NACPs within patterned PACPs. Time-lapse images over one oscillation cycle with PDMS clusters within an arrayed pattern of 10 μm polystyrene beads. (ii) Experimental demonstration of selective navigation of a PDMS cluster through a locally stationary polystyrene-bead lattice. ( D ) Schematics and experiments showing that STAT enables (i) gentle oscillation of biological cells and (ii) controllable transport of NACPs while maintaining cells in a patterned lattice. In (B) to (D), the polystyrene beads, PDMS clusters, and K562 cells are highlighted in red, blue, and light green, respectively. Oscillation was captured at ω x /2π = 20.094 MHz and ω y /2π = 20.094 MHz − 1 Hz, corresponding to Δω = 1 × 2π Hz, when P x > P y . In (C) and (D), black and green arrows represent transport and oscillation directions, respectively. Scale bars, 45 μm.

Article Snippet: K562 suspension cells and B16-F10 adherent melanoma cells were cultured in RPMI 1640 [American Type Culture Collection (ATCC), 30-2001] and Dulbecco’s modified Eagle’s medium (ATCC, 30-2002), respectively.

Techniques: High Throughput Screening Assay, Shear, Gentle

Journal: Science Advances

Article Title: Space-time acoustofluidic tweezers for dynamic and selective manipulation of microparticles

doi: 10.1126/sciadv.aee2983

Figure Lengend Snippet: ( A ) Images of K562 cells, showing a half-cycle oscillation with ζ x and ζ y denoting oscillation displacements in x and y . ( B ) Measured cell oscillation displacements as functions of frequency detuning Δω. Green bars show ζ x , while blue bars indicate ζ y . ( C ) Measured cell oscillation displacements as functions of driving voltage amplitudes V x when V y = 11 V pp and V y when V x = 11 V pp . ( D ) Schematics illustrating how tuning Δ P and Δω controls the transport of a PDMS cluster between a lattice pattern of K562 cells, showing gentle oscillations around stable pressure nodes. ( E ) Time-elapse images of tuning Δω and Δ P to selectively manipulate a PDMS cluster through an effectively stationary K562 cell pattern.

Article Snippet: K562 suspension cells and B16-F10 adherent melanoma cells were cultured in RPMI 1640 [American Type Culture Collection (ATCC), 30-2001] and Dulbecco’s modified Eagle’s medium (ATCC, 30-2002), respectively.

Techniques: Gentle